Biocompatibility study of combined pH-responsive polymeric matrices with application in Bone Tissue Engineering
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Abstract
In the present work, the preparation of a biomaterial from a synthetic terpolymer (TerP) and a natural polymer, physically crosslinked, is shown. In order to evaluate the new material for bone tissue regeneration, physicochemical and biological characterizations were performed. The membranes were obtained by solvent casting and characterized using FTIR spectroscopy, swelling tests, contact angle measurements, and scanning electron microscopy (SEM). It was found that the compatibility between the polymers is stable at physiological pH and the incorporation of a higher amount of TerP into the matrix increases hydrophobicity and porosity.Furthermore, considering the intended application of these materials, studies of biocompatibility and cytotoxicity were conducted with Bone Marrow Progenitor Cells (BMPCs) and RAW264.7 cells, respectively. Cell proliferation, NO production and release into the culture medium for 24 and 48 hours, and proinflammatory cytokine expression of IL-1β and TNF-α from cells grown on the biomaterials while varying the amount of the synthetic polymer were evaluated. Greater cell proliferation and lower NO production were found on matrices containing a lower proportion of TerP, in addition to better biocompatibility. The results of this study demonstrate that the obtained terpolymer and its combination with a natural polymer is a highly interesting strategy for biomaterial preparation with potential applications in regenerative medicine. This approach could be extended to other structurally related systems.
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Derechos de autor: Actualizaciones en Osteología es la revista oficial de la Asociación Argentina de Osteología y Metabolismo Mineral (AAOMM) que posee los derechos de autor de todo el material publicado en dicha revista.
References
Azi ML, Aprato A, Santi I, Kfuri M, Masse A, Joeris A. Autologous bone graft in the treatment of post-traumatic bone defects: a systematic review and meta-analysis. BMC Musculoskeletal Disord 2016;17:465.
Fernández JM. Importancia de la angiogénesis en el diseño de scaffolds para ingeniería de tejido óseo. Actual Osteol 2020;16:211-31.
Shichman I, Roof M, Askew N, et al. Projections and Epidemiology of Primary Hip and Knee Arthroplasty in Medicare Patients to 2040-2060. JB JS Open Access 2023;8:e22.00112.
Rather HA, Jhala D, Vasita R. Dual functional approaches for osteogenesis coupled angiogenesis in bone tissue engineering. Material Sci Eng C 2019;103:109761.
Prina R, Rubino P, Gutiérrez R, Cassini F. Resultados tempranos de la artroplastia cervical utilizando Prodisc-C. Rev Argent Neurocir 2009;23:59-64.
Villena DS, Sotelano P, Conti L y cols. Comparación de los resultados de la artroplastia total de tobillo en pacientes ≤55 y >55 años. Rev Asoc Argent Ortop Traumatol 2020;85:305-16.
Loures FB, Chaoubah A, Oliveira VM de, Almeida AM, Campos EM de S, Paiva E P de. Economic analysis of surgical treatment of hip fracture in older adults. Revista de Saúde Pública 2015;49:12-9.
Sánchez A. El caballero y la dama con osteoporosis. Actual Osteol 2010;6:81-9.
LeBoff MS, Greenspan SL, Insogna KL et al. The clinician's guide to prevention and treatment of osteoporosis. Osteoporos Int 2022;33:2049-102.
Sobh MM, Abdalbary M, Elnagar S, et al. Secondary Osteoporosis and Matabolic Bone Disease. Journal of Clinical Medical 2022;11(9):2382.
McCarthy AD, Molinuevo MS, Cortizo AM. AGEs and Bone ageing in Diabetes mellitus. J Diabetes Metab 2013;4:276.
Chen Y, Zhou Y, Lin J, Zhang S. Challenges to Improve Bone Healing Under Diabetic Conditions. Front Endocrinol (Lausanne) 2022;13:861878.
Schurman L, Bagur A, Claus-Hermberg H y cols. Guías 2012 para el diagnóstico, la prevención y el tratamiento de la osteoporosis. Actual Osteol 2013;9:123-53.
Schurman L, Galich A, González C y cols. Guías argentinas para el diagnóstico, la prevención y el tratamiento de la osteoporosis 2015. Actual Osteol 2017;13:136-56.
Lu YC, Lin YC, Lin YK, et al. Prevalence of osteoporosis and low bone mass in older chinese population based on bone mineral density at multiple skeletal sites. Scie Rep- UK 2016;6:25206.
Langer R, Vacanti JP. Tissue Engineering. Science 1993;260:920-6.
Benmassaoud MM, Gultian KA, DiCerbo M, Vega SL. Hydrogel screening approaches for bone and cartilage tissue regeneration. Ann NY Acad Sci 2020;1460:25-42.
Alfano AL, Fernández JM. Induction of Topographical Changes in Poly-e-Caprolactone Scaffolds for Bone Tissue Engineering: Biocompatibility and Cytotoxicity Evaluations. J Biomater Tissue Eng 2015;5:142-9.
Belluzo MS, Medina LF, Cortizo AM, Cortizo MS. Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications. Ultrason Sonochem 2016;30:1-8.
Belluzo MS, Medina LF, Molinuevo MS, Cortizo MS, Cortizo AM. Nanobiocomposite based on natural polyelectrolytes for bone regeneration. J Biomed Mater Res A 2020;108:1467-78.
BraviCostantino ML, Oberti TG, Cortizo AM, Cortizo MS. Matrices based on lineal and star fumarate-metha/acrylate copolymers for bone tissue engineering: Characterization and biocompatibility studies. J Biomed Mater Res Part A 2019;107A:195-203.
BraviCostantino ML, Cortizo MS, Cortizo AM, Oberti TG. Osteogenic scaffolds based on fumaric/N-isopropylacrylamide copolymers: Designed, properties and biocompatibility studies. Eur Polym J. 2020;122:109348.
Cortizo MS, Molinuevo MS, Cortizo AM. Biocompatibility and biodegradation of polyester and polyfumarate based-scaffolds for bone tissue engineering. J Tissue Eng Regen Med 2008;2:33-42.
Fernández JM, Molinuevo MS, Cortizo AM, McCarthy AD, Cortizo MS. Characterization of poly(epsilon-caprolactone)/polyfumarate blends as scaffolds for bone tissue engineering. J Biomater Sci Polymer 2010;21:1297-312.
Fernández JM, Cortizo MS, Cortizo AM. Fumarate/Ceramic Composite Based Scaffolds for Tissue Engineering: Evaluation of Hydrophylicity, Degradability, Toxicity and Biocompatibility. J BiomaterTissue Eng 2014;4:227-34.
Fernández JM, Oberti TG, Vikingsson L, Gómez Ribelles JL, Cortizo AM. Biodegradable polyester networks including hydrophilic groups favor BMSCs differentiation and can be eroded by macrophage action. Polym Degrad Stabil 2016; 130:38-46.
Lastra ML, Molinuevo MS, Cortizo AM, Cortizo MS. Fumarate Copolymer-Chitosan Cross-Linked Scaffold Directed to Osteochondrogenic Tissue Engineering. Macromol Biosci 2017;17.
Lastra ML, Molinuevo MS, Blaszczyk-Lezak I, Mijangos C, Cortizo MS. Nanostructured fumarate copolymer-chitosan crosslinked scaffold: An in vitro osteochondrogenesis regeneration study. J Biomed Mater Res A 2018;106:570-9.
Lino AB, McCarthy AD, Fernández JM. Evaluation of Strontium-Containing PCL-PDIPF Scaffolds for Bone Tissue Engineering: In Vitro and In Vivo Studies. Ann Biomed Eng 2019;47:902-12.
Torres ML, Fernández JM, Dellatorre FG, Cortizo AM, Oberti TG. Purification of alginate improves its biocompatibility and eliminates cytotoxicity in matrix for bone tissue engineering. Algal Res 2019;40:101499.
Torres ML, Oberti TG, Fernández JM. HEMA and alginate-based chondrogenic semi-interpenetrated hydrogels: synthesis and biological characterization. Biomater Sci Polym Ed 2021;32:504-23.
BraviCostantino ML, Belluzo MS, Oberti TG, Cortizo AM, Cortizo MS. Terpolymer-Chitosan membranes as biomaterial. J Biomed Mater Res Part A 2022;110:2383-93.
Oberti TG, Cortizo MS, Alessandrini JL. Novel copolymer of diisopropyl fumarate and benzyl acrylate synthesized under microwave energy and quasielastic light scattering measurements. J Macromol SCI: Part A 2010;47:725- 31.
Demirci S, Alaslan A, Caykara T. Preparation, characterization and surface pKa values of poly (N-vinyl-2-pyrrolidone)/chitosan blend films. Appl Surf Sci 2009;255;5979-83.
Bondaz L, Cousin F, Muller F, et al. pH sensitive behavior of the PS-b-PDMAEMA copolymer at the air-water interface. Polymer 2021;221:123619.
Molinuevo MS, Schurman L, McCarthy AD, et al. Effect of metformin on bone marrow progenitor cell differentiation: in vivo and in vitro studies. J Bone Miner Res 2010;25: 211-21.
Lino AB, Fernández JM, Molinuevo MS, Cortizo AM, McCarthy AD. Efectos in vivo del ranelato de estroncio sobre células progenitoras de médula ósea de ratas diabéticas. Actual Osteol 2016;12:78-86.
Cortizo AM, Ruderman G, Correa G, Mogilner IG, Tolosa EJ. Effect of Surface Topography of Collagen Scaffolds on Cytotoxicity and Osteoblast Differentiation. J Biomater Tiss Eng. 2012;2:125-32.
Nagud A, Alghfeli L, Elmasry M, El-Serafi I, El-Serafi A. Biomaterials as a Vital Frontier for Stem Cell-Based Tissue Regeneration. Front Cell Dev Biol. 2022;10: article 713934.
Zaveri TD, Dolgova NV, Chu BH, et al. Contributions of surface topography and cytotoxicity to the macrophage response to zinc oxide nanorods. Biomaterials 2010; 31:2999-3007.
Cun X, Hosta-Rigau L. Topography: A Biophysical Approach to Direct the Fate of Mesenchymal Stem Cells in Tissue Engineering Applications. Nanomaterials (Basel) 2020;10:207.
Lastra ML, Molinuevo MS, Giussi JM, et al. Tautomerizable β-ketonitrile copolymers for bone tissue engineering: Studies of biocompatibility and cytotoxicity. Mat Scie Eng C.2015;51:256-62.